In human locomotion, we continuously modulate joint mechanical impedance of the lower limb (hip, knee, and ankle) either voluntarily or reflexively to accommodate environmental changes and maintain stable interaction. Ankle mechanical impedance plays a pivotal role at the interface between the neuro-mechanical system and the physical world. We characterized human ankle mechanical impedance in two degrees-of-freedom simultaneously using an ankle exoskeleton robot, the Anklebot. Static, dynamic impedance at rest posture and time-varying impedance during walking were estimated in previous studies, and we are extending the work towards rehabilitation.